Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/11/2021 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
COSTA, R. R. da; SILVA, M. R.; AUGUSTO, C. J.; LEITE, I. C. G. |
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Afiliação: |
RONALDO RODRIGUES DA COSTA, Universidade Federal de Juiz de Fora; MARCIO ROBERTO SILVA, CNPGL; CLAUDIO JOSE AUGUSTO, Fundação Ezequiel Dias; ISABEL CRISTINA GONÇALVES LEITE, Universidade Federal de Juiz de Fora. |
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Título: |
Fast, simple and cheap: method modified from conventional cultivation for tuberculosis diagnosis allows seeding on Löwenstein-Jensen of any swab-embedded pulmonary samples decontaminated with sodium hydroxide. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Transactions of The Royal Society of Tropical Medicine and Hygiene, trab162, 2021. |
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DOI: |
https://doi.org/10.1093/trstmh/trab162 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Few tuberculosis (TB) control programmes in low-income countries have access to culture facilities in their primary care diagnostic centres and this scenario may have worsened with the coronavirus disease 2019 pandemic. Thus the aim was to develop and evaluate a simpler TB test that allows seeding on Löwenstein?Jensen (LJ) medium of several swab-embedded samples decontaminated with sodium hydroxide (NaOH). Methods: A cotton swab containing each sample was decontaminated in NaOH before being dipped into aslightly acidic solution to neutralize the pH in order to allow the culture to develop on LJ medium. Samples (n=543) from suspected or confirmed pulmonary TB were analysed in two phases: standardization (n=167) and evaluation of the study method (n=376). Results: The study method showed sensitivity >95% and specificity >93% using Ogawa?Kudoh (OK) and modified Petroff (MP) as standards and was comparable to MP-LJ (p>0.05) and slightly superior to OK (p=0.03) for sputum culture and more comprehensive than the latter for other pulmonary specimens. Conclusions: This article reports a more comprehensive, simpler and less costly method for diagnosing TB inthe laboratory with fewer economic resources and biosafety equipment. Thus a patent application was filed (BR1020190103841). |
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Thesagro: |
Doença Animal; Tuberculose. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227661/1/Fast-simple.pdf
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Marc: |
LEADER 02062naa a2200193 a 4500
001 2136041
005 2021-11-11
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1093/trstmh/trab162$2DOI
100 1 $aCOSTA, R. R. da
245 $aFast, simple and cheap$bmethod modified from conventional cultivation for tuberculosis diagnosis allows seeding on Löwenstein-Jensen of any swab-embedded pulmonary samples decontaminated with sodium hydroxide.$h[electronic resource]
260 $c2021
520 $aBackground: Few tuberculosis (TB) control programmes in low-income countries have access to culture facilities in their primary care diagnostic centres and this scenario may have worsened with the coronavirus disease 2019 pandemic. Thus the aim was to develop and evaluate a simpler TB test that allows seeding on Löwenstein?Jensen (LJ) medium of several swab-embedded samples decontaminated with sodium hydroxide (NaOH). Methods: A cotton swab containing each sample was decontaminated in NaOH before being dipped into aslightly acidic solution to neutralize the pH in order to allow the culture to develop on LJ medium. Samples (n=543) from suspected or confirmed pulmonary TB were analysed in two phases: standardization (n=167) and evaluation of the study method (n=376). Results: The study method showed sensitivity >95% and specificity >93% using Ogawa?Kudoh (OK) and modified Petroff (MP) as standards and was comparable to MP-LJ (p>0.05) and slightly superior to OK (p=0.03) for sputum culture and more comprehensive than the latter for other pulmonary specimens. Conclusions: This article reports a more comprehensive, simpler and less costly method for diagnosing TB inthe laboratory with fewer economic resources and biosafety equipment. Thus a patent application was filed (BR1020190103841).
650 $aDoença Animal
650 $aTuberculose
700 1 $aSILVA, M. R.
700 1 $aAUGUSTO, C. J.
700 1 $aLEITE, I. C. G.
773 $tTransactions of The Royal Society of Tropical Medicine and Hygiene, trab162, 2021.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
